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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
13/12/2004 |
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Data da última atualização: |
13/12/2004 |
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Autoria: |
ABDELNOOR, R. V. |
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Título: |
Cloning and characterization of MSH1 in higher plants and its involvment in regulation of substoichiometric shifting. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
2004. |
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Páginas: |
123 f. |
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Idioma: |
Inglês |
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Notas: |
Dissertation (Doctor of Phylosophy in Agronomy - Plant Breeding and Genetics) - University of Nebraska, Lincoln. |
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Conteúdo: |
Plant mitochondrial genomes are much larger and complex in structure than in other euraryotes. The high recombinational activity of these genomes produces sub-genomic molecules of different sizes that can be maintained in a variable copy number, a phenomenon that is called substoichiometriv shifting and is widespread in plants. The regulation of the copy number has been shown to be under nuclear control, but the mechanism of this regulation is still not well understood. CHM is a nuclear gene that influences the mitochondrial genome by modifying the relative copy number of some sub-genomic molecules in Arabidopsis. Mutations in this gene are characterized by green-white variegation, abnormal growth, distorted leaves and, insome cases cytoplasmic male sterility. The main objectives of this thesis are to map, clone and characterize the CHM locus in Arabidopsis and its orthologs in other plant species. The CHM mapping was done with F2 populations involving the mutant chm1-1. A candidate gene was identified, designated AtMSH1m which is homologous ti the MutS protein of E. coli that is involved in DNA mismatch repair. The identity of this gene was confirmed by T-DNA insertional mutagenesis and by sequencing the mutant alleles for the locus. To better understand the structure and function of MHS1 in plants, the full-length orthologous genes in rice, tomato, maize, soybean and common bean were isolated and sequenced. Multiple alignment and phylogenetic analysis allowed the postulation of the origin of this gene and the identification of specific domains that could be very important for their proper function in plant mitochondria, including an endonuclease domain on the c-terminus of MSH1. This endonuclease is unique to palnt MSH1 and may be responsible for some of the exclusive properties of this protein. Also, a model for the function of MSH1 in the regulation of substoichiometric shifting is presented. MenosPlant mitochondrial genomes are much larger and complex in structure than in other euraryotes. The high recombinational activity of these genomes produces sub-genomic molecules of different sizes that can be maintained in a variable copy number, a phenomenon that is called substoichiometriv shifting and is widespread in plants. The regulation of the copy number has been shown to be under nuclear control, but the mechanism of this regulation is still not well understood. CHM is a nuclear gene that influences the mitochondrial genome by modifying the relative copy number of some sub-genomic molecules in Arabidopsis. Mutations in this gene are characterized by green-white variegation, abnormal growth, distorted leaves and, insome cases cytoplasmic male sterility. The main objectives of this thesis are to map, clone and characterize the CHM locus in Arabidopsis and its orthologs in other plant species. The CHM mapping was done with F2 populations involving the mutant chm1-1. A candidate gene was identified, designated AtMSH1m which is homologous ti the MutS protein of E. coli that is involved in DNA mismatch repair. The identity of this gene was confirmed by T-DNA insertional mutagenesis and by sequencing the mutant alleles for the locus. To better understand the structure and function of MHS1 in plants, the full-length orthologous genes in rice, tomato, maize, soybean and common bean were isolated and sequenced. Multiple alignment and phylogenetic analysis allowed the postulat... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02416nam a2200133 a 4500
001 1467331
005 2004-12-13
008 2004 bl uuuu m 00u1 u #d
100 1 $aABDELNOOR, R. V.
245 $aCloning and characterization of MSH1 in higher plants and its involvment in regulation of substoichiometric shifting.
260 $a2004.$c2004
300 $a123 f.
500 $aDissertation (Doctor of Phylosophy in Agronomy - Plant Breeding and Genetics) - University of Nebraska, Lincoln.
520 $aPlant mitochondrial genomes are much larger and complex in structure than in other euraryotes. The high recombinational activity of these genomes produces sub-genomic molecules of different sizes that can be maintained in a variable copy number, a phenomenon that is called substoichiometriv shifting and is widespread in plants. The regulation of the copy number has been shown to be under nuclear control, but the mechanism of this regulation is still not well understood. CHM is a nuclear gene that influences the mitochondrial genome by modifying the relative copy number of some sub-genomic molecules in Arabidopsis. Mutations in this gene are characterized by green-white variegation, abnormal growth, distorted leaves and, insome cases cytoplasmic male sterility. The main objectives of this thesis are to map, clone and characterize the CHM locus in Arabidopsis and its orthologs in other plant species. The CHM mapping was done with F2 populations involving the mutant chm1-1. A candidate gene was identified, designated AtMSH1m which is homologous ti the MutS protein of E. coli that is involved in DNA mismatch repair. The identity of this gene was confirmed by T-DNA insertional mutagenesis and by sequencing the mutant alleles for the locus. To better understand the structure and function of MHS1 in plants, the full-length orthologous genes in rice, tomato, maize, soybean and common bean were isolated and sequenced. Multiple alignment and phylogenetic analysis allowed the postulation of the origin of this gene and the identification of specific domains that could be very important for their proper function in plant mitochondria, including an endonuclease domain on the c-terminus of MSH1. This endonuclease is unique to palnt MSH1 and may be responsible for some of the exclusive properties of this protein. Also, a model for the function of MSH1 in the regulation of substoichiometric shifting is presented.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
15/05/2013 |
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Data da última atualização: |
15/05/2013 |
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Tipo da produção científica: |
Circular Técnica |
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Autoria: |
OLIVEIRA, V. M. de. |
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Afiliação: |
VANIA MARIA DE OLIVEIRA, CNPGL. |
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Título: |
Diagnóstico da mastite bovina. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Juiz de Fora: EMBRAPA-CNPGL-ADT, 1998. |
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Páginas: |
24 p. |
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Descrição Física: |
il. |
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Série: |
(EMBRAPA-CNPGL. Circular técnica, 51). |
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Idioma: |
Português |
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Conteúdo: |
Sintomas da mastite; Clinica; Subclinica; Celulas somaticas - definicao; Diagnostico da mastite clinica; Exame do ubere; Exame visual (antes da ordenha); Exame manual do ubere e tetas (apos a ordenha); Teste da caneca de fundo escuro ou caneca telada; Diagnostico diferencial da mastite; Lesoes das tetas; Diagnostico da mastite subclinica; Metodos de deteccao das celulas somaticas; CCS atraves de contadores eletronicos; Contagem direta em laminas (CDL); California Mastitis Test (CMT); Teste do Viscosimetro (WMT); Teste para medir a condutividade eletrica; Teste com o "Detector de Condutividade" manual; Sistema de condutividade acoplado a ordenhadeira mecanica; Como calcular o nivel de mastite em um rebanho; Mastite clinica; Mastite subclinica; Exames microbiologicos; Taxa de cloretos - nota esclarecedora. |
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Palavras-Chave: |
Bovine; Bovinos; Control; Controle; Diagnosis; Disease; Enfermidade; Exames; Gado de leite; Mamimary gland diseases; Mastite; Mastite bovina; Symptions. |
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Thesagro: |
Animal; Bovino; Diagnostico; Doença; Doença Animal; Gado Leiteiro; Infecção; Mamite; Profilaxia; Sintoma. |
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Thesaurus NAL: |
animal diseases; cattle; dairy cattle; infection; mastitis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/82845/1/Diagnostico-da-mastite-bovina.pdf
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Marc: |
LEADER 01940nam a2200469 a 4500
001 1957987
005 2013-05-15
008 1998 bl uuuu u0uu1 u #d
100 1 $aOLIVEIRA, V. M. de
245 $aDiagnóstico da mastite bovina.$h[electronic resource]
260 $aJuiz de Fora: EMBRAPA-CNPGL-ADT$c1998
300 $a24 p.$cil.
490 $a(EMBRAPA-CNPGL. Circular técnica, 51).
520 $aSintomas da mastite; Clinica; Subclinica; Celulas somaticas - definicao; Diagnostico da mastite clinica; Exame do ubere; Exame visual (antes da ordenha); Exame manual do ubere e tetas (apos a ordenha); Teste da caneca de fundo escuro ou caneca telada; Diagnostico diferencial da mastite; Lesoes das tetas; Diagnostico da mastite subclinica; Metodos de deteccao das celulas somaticas; CCS atraves de contadores eletronicos; Contagem direta em laminas (CDL); California Mastitis Test (CMT); Teste do Viscosimetro (WMT); Teste para medir a condutividade eletrica; Teste com o "Detector de Condutividade" manual; Sistema de condutividade acoplado a ordenhadeira mecanica; Como calcular o nivel de mastite em um rebanho; Mastite clinica; Mastite subclinica; Exames microbiologicos; Taxa de cloretos - nota esclarecedora.
650 $aanimal diseases
650 $acattle
650 $adairy cattle
650 $ainfection
650 $amastitis
650 $aAnimal
650 $aBovino
650 $aDiagnostico
650 $aDoença
650 $aDoença Animal
650 $aGado Leiteiro
650 $aInfecção
650 $aMamite
650 $aProfilaxia
650 $aSintoma
653 $aBovine
653 $aBovinos
653 $aControl
653 $aControle
653 $aDiagnosis
653 $aDisease
653 $aEnfermidade
653 $aExames
653 $aGado de leite
653 $aMamimary gland diseases
653 $aMastite
653 $aMastite bovina
653 $aSymptions
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Embrapa Gado de Leite (CNPGL) |
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